Rewatch this 3P-Seminar
‘Extracellular vesicles are small organelles released in the cellular milieu by almost any kind of cells. Their lipid/RNA/protein content varies upon different stimuli inside the cells. The GGGGCC expansion in the C9orf72 gene is the most common ALS mutation. This expansion is aberrantly translated into five dipeptide proteins which are included in the EVs and are known to be toxic in neuronal cells. In my talk I am going to discuss the role of the EVs in seeding toxicity between neighbor cells and which are aberrant mechanisms that lead to cell death in recipient cells’.
‘Hallmarks of Parkinson’s disease (PD) include Lewy-body inclusions and immune alterations in both the innate and adaptive immune system. In PD, altered populations of T cells exist and T cells recognize epitopes derived from α-syn. To study the role of T cells with respect to α-syn pathology, we injected human α-syn preformed fibrils (PFFs) into the striatum of immunocompromised mice (NSG) and assessed accumulation of phosphorylated α-syn and microgliosis. NSG mice, compared to wildtype mice, had increased phosphorylated α-syn pathology in the substantia nigra. Reconstitution of wildtype T cells into NSG mice, decreased the accumulation of α-syn pathology, increased microgliosis, when compared to non-transplanted mice.’